| Communication contains the immunology data treatment. New nonlinear methods of immunofluorescence statistical analysis of peripheral blood neutrophils have been developed. We used technology of respiratory burst reaction of DNA fluorescence in the neutrophils cells nuclei due to oxidative activity. The histograms of photon count statistics of the radiant neutrophils populations' in flow cytometry experiments are considered. Distributions of the fluorescence flashes frequency as functions of the fluorescence intensity are analyzed. Statistic peculiarities of histograms set allow divide all histograms into three classes. The classification is based on three different types of smoothing and long-range scale averaged the immunofluorescence distributions with maximum, minimum and monotonic change. First histograms group belongs to healthy donors. Two other groups belong to donors with inflammatory and autoimmune diseases. Some of the illnesses are not diagnosed by standards biochemical methods. Dynamics of a change in the immunofluorescence distributions of healthy and sick people in the process of medical treatment are examined; we observed the right and the contrary transitions or coup of the averaged distributions in histograms. Peculiarities of immunofluorescence for women in pregnant period are classified. Health or illness criteria are connected with statistics features of immunofluorescence histograms. Neutrophils populations' fluorescence presents the sensitive clear indicator of health status. |
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