| Column is the most critical part of the chromatographic separation. Monolithic column is a novel chromatographic column. The development of monolithic column significantly affects the development of chromatographic analysis. Monolithic column possesses high column efficiency and can reduce the analysis time as the permeability efficiency improves. Especially in protein separation, the superior and unique porous structure of monolithic column could enhance the permeability of proteins separation and improve separation efficiency. Recently, monolithic columns are developing rapidly due to their fascinating properties and enormous application. In this paper, recent development and application of monolithic columns are reviewed, which will deepen people.s understanding of this field and have profound implications for future design of high-efficiency monolithic column. |
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