|
1 Analytical Biotechnology, TU-Delft, Julianalaan 67, 2628 BC Delft, The Netherlands
2 NovemberAG, Ulrich-Schalk-Str. 3D-91056 Erlangen, Germany
3 Institut fuer Experimentalphysik, Karl Franzens Universitaet,
Universitaetsplatz 3, A - 8010 Graz, Austria
4 Institut fuer Biochemie und molekulare Zellbiologie, Universitaet Wien,
1030 Wien, Dr. Bohrgasse 9, Austria
Correspondence to:
Christian Mayer, TU-Delft, ABT, Julianalaan 67, 2628BC Delft, The Netherlands
email: C.Mayer@tnw.tudelft.nl,
Fax: 0031-15-278-2355
Key Words : nano cluster layers, fluorescence enhancement, biochip, palladium
| Structural enhanced fluorescence (SEF) amplifies fluorescent signals 200 fold in comparison to standard glass slides. The signal-boosting phenomenon is ideally suited for sensitive biochip applications. DNA biochips based on hybridisation analysis need to cope with chemical and thermal stress, therefore a stable multilayer set up and chemically active surface layers were invented. A palladium layer introduces chemical stability and the necessary structural compatibility for the SEF technique. A silver cluster layer enables the excitation of surface plasmons; sandwich layers of metallic glass establish the proper distance to the fluorophores. The chips are compatible with standard assay procedures of DNA-biochips, and can be reused after hybridisation by stripping in boiling water. |
full paper (pdf, 96 Kb)