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State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry & Chemical
Engineering, Institute of biological Science and Biological Technology,
Hunan University, Changsha 410082, P.R.China
| We have developed a novel method for efficient gene delivery using amino-modified silica coated magnetic nanoparticles. The positively charged amino-modified silica coated magnetic nanoparticles have been directly prepared using the synchronous hydrolysis of tetraethoxysilane and N-(β-amimoethyl)- γ-aminopro- pyltriethoxysilane in water-in-oil microemulsion after the synthesis of aqueous magnetic ferrofluid with the precipitation of the chloride mixture with the base. Plasmid DNA can be easily combined onto the positively charged nanoparticle surface to form Nanoparticles-plasmid DNA complexes. The combined plasmid DNA was effectively protected from enzymatic digestion of DNaseI. And the nanoparticles-plasmid DNA complexes can successfully cross various systemic barriers to COS-7 cells as well as mediate high expression of Green Fluorescence Protein (GFP) gene in cells by use of this new amino-modified silica coated magnetic nanoparticles as gene delivery. It would provide a new technology and measure for gene expression, gene filtration, gene function research and gene therapy. |
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